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Electrophoresis was run for 30 min on an 1 % agarose gel.
AGE, agarose gel electrophoresis) je metoda ločevanja DNK po velikosti v agaroznem gelu in pod vplivom delovanja električnega polja.
Vzorcem smo dodali nanašalni pufer (6x agarose gel loading buffer, Fermentas) in jih nanesli na gel.
As visualised under UV-light after agarose gel electroforesis.
Medij in celični lizat sta bila preoblikovana z uporabo čistilnega kompleta Qiagen Nickel - agarose .
Figure 1: Agarose gel electrophoresis of DNA from eight samples of himalayan soil retrieved in year 2002.
Agarose gel electrophoresis Za ugotavljanje uspešnosti PCR pomnoževanja smo PCR pomnožke najprej ločili s horizontalno agarozno gelsko elektroforezo.
DNA was visualized with gel electrophoresis on 0.7% agarose in TAE buffer at 80 V for 1.5 h.
It was not possible to clearly visualize plasmid DNA on agarose gels in the case of L. brevis MB124.
Figure 23: Separation of linearized fragments of vectors pRF-HU2 on an agarose gel.
Figure 8: Agarose gel electrophoresis before and after restriction with restriction enzyme Taq1.
Figure 26: Agarose electrophoresis (left) and agarose gel (right) with PCR amplicons.
Figure 4.10: Example of agarose gel electrophoresis of RT - PCR amplification of flaA of C. jejuni cells after exposure to different stresses.
Ta je shranjena v pufru, zato smo potrebovali 1912,5 µL agaroze (EKaptureTM Agarose).
The presence of plasmid DNA in L. brevis MB124 could not be confirmed on agarose gels.
Dodali smo 1,72 g agaroze (Agarose SPI, Duchefa Biochemie) in mešanico segreli v mikrovalovni pečici, da se je agaroza povsem raztopila.
Figure 8: Analysis of RT-PCR results on a 2 % agarose gel for hops infected with CBCVd using injection of viroid inoculum 120 dpi.
Nespecifično vezani proteini so se pri nizki koncentraciji imidazola (10 mM) eluirali iz kolone (Ni-NTA Agarose, 2020).
Figure 35: Agarose gel electrophoresis of amplified target regions of the FRIGIDA gene in oilseed rape (A) and cabbage (B).
Agarose Gel Electrophoresis) bazni par goveji serumski albumin (angl.